Job ID: 36075
Location: Vancouver - Other
Employment Group: Technicians & Research Assists
Job Category: Non Union Research/Technical
Classification Title: Research Asst/Tech 3
Business Title: Research Asst/Tech 3
VP/Faculty: Faculty of Medicine
Department: Ctr-Molecular Med&Therapeutics
Salary Range: $45,035.00 (minimum) - $49,112.00 (midpoint) - $53,190.00 (maximum)
Full/Part Time: Full-Time
Desired Start Date: 2019/12/09
Job End Date: 2020/12/08
Funding Type: Grant Funded
Other: Leave Replacement
Closing Date: 2019/12/09
Available Openings: 1
Guiding principle: "Midpoint" of the hiring salary range means the individual possesses full job knowledge, qualifications and experience.
Job Summary
This is a one year maternity leave replacement position with the Mouse Animal Production Service (MAPS) team.
The main responsibilities of this position are maintaining MAPS mouse colonies, tissue culture, PCR, and all aspects of MAPS projects and procedures including sperm cryopreservation and recovery, minor surgeries, in-vitro fertilization, and ES cell and RNA CRISPR microinjection. In this position, you will be required to prepare marketing materials, and communicate with potential and existing MAPS clients via email and in person. Someone that works well in a team is essential, with exceptional client skills, but most technical work is done independently. Some evening and weekend work is required.
Organizational Status
The position reports to Dr. Elizabeth M. Simpson, Director of MAPS. The employee will work in a laboratory affiliated with the University of British Columbia, and subject to the universities workplace safety rules. The workspace includes a molecular biology laboratory and a barrier facility in the basement of the building. Wearing of specialized clothing and daily use of an air shower for decontamination are required.
Work Performed
. Microinjection of mouse embryonic stem cells (ESCs) and DNA/RNA into fertilized mouse embryos utilizing highly specialized equipment
. Harvesting mouse embryos and oocytes at various stages of development
. Sperm cryopreservation and IVF recovery
. Minor surgical procedures (vasectomies, embryo transfer)
. Mouse colony maintenance and breeding including: husbandry, breeding schemes, planning experiments, weaning, ear notching and running PCR, setting up natural and timed pregnant matings
. Monitoring the health of mice
. Update mouse colony records and inventory: excel sheet, and online database
. Sub Q and IP injections in mice
. Writing SOPs and updating protocols as needed
. Cell culture: preparation of media, feeding and expanding mouse ESCs, preparation of feeder cells, freezing and thawing cells for culture, performing stem cell electroporations.
. Attendance at a weekly lab meeting
. Attendance at customer meetings
. Creating and distributing marketing materials
. Communicating with administrative staff for billing and quote purposes
. Maintaining supplies inventories, and communicating to administrative staff order details
. Writing and preparing project quotes
. Molecular biology specific to CRISPR including designing guides and donors, complexing guides and Cas9 for microinjection and/or electroporation, and designing PCR and sequencing assays to confirm editing events.
Supervision Received
This position will be supervised by Dr. Elizabeth Simpson
Supervision Given
No supervisory role
Consequence of Error/Judgement
In general, the work described follows well-established protocols and techniques. Adherence to these protocols is mandatory for the success of the projects. However, the ability to demonstrate proper judgment and accountability is essential. Errors will likely be non-critical but will require the task be performed again correctly and may cause delays in meeting project goals.
Qualifications
Undergraduate degree in a relevant discipline or Graduation from a technical college or institute. Animal Health Technologist diploma and RLAT (or RLATR) CALAS certification are assets.
University degree in relevant field (biochemistry, genetics, molecular biology) required, M.Sc. or higher preferred Minimum of 3 years related experience or the equivalent combination of education and experience. A minimum of 3 years related animal experience. Ability to maintain accuracy and attention to detail.
Excellent organizational skills and thoroughness are a must. Ability to prioritize and work effectively under pressure to meet deadlines. Proven ability to effectively manage multiple tasks and priorities, while remaining flexii. Ability to perform research-related procedures (e.g., prepare solutions) (e.g., dissecting) (e.g., prepare specimens).
Applicants must have experience working with rodents. Molecular biology experience and tissue culture experience are assets. Ability to work effectively independently and in a team environment. Ability to exercise tact and discretion. Ability to develop and maintain cooperative and productive working relationships. Ability to effectively use <job-specific software> at an intermediate level. (e.g., Outlook, MS Word, MS Excel)
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Equity and diversity are essential to academic excellence. An open and diverse community fosters the inclusion of voices that have been underrepresented or discouraged. We encourage applications from members of groups that have been marginalized on any grounds enumerated under the B.C. Human Rights Code, including sex, sexual orientation, gender identity or expression, racialization, disability, political belief, religion, marital or family status, age, and/or status as a First Nation, Metis, Inuit, or Indigenous person.
All qualified candidates are encouraged to apply; however Canadians and permanent residents will be given priority.
